<asp:RegularExpressionValidator
id="FileUpLoadValidator" runat="server"
ErrorMessage="Upload Jpegs and Gifs only."
ValidationExpression="^(([a-zA-Z]:)|(\\{2}\w+)\$?)(\\(\w[\w].*))(.jpg|.JPG|.gif|.GIF)$"
ControlToValidate="FileUpload1">
</asp:RegularExpressionValidator>